The Hormone and Urinary Metabolites Assessment Profile (HuMap™) provides a comprehensive overview of steroid hormones, their metabolites, and the efficiency of the enzymes that metabolize these hormones. This non-invasive test requires only 4 or 5 separate urine collections. Because the breakdown of hormones relies so heavily on processes within the liver, this test can also help reveal patterns of liver metabolism and conjugation. Additionally, testing urinary hormone metabolites can contribute to further understanding of endogenous hormone secretion, supplemental hormone utilization, enzyme activity, oxidative stress, and insight into how your body metabolizes hormones.

This panel contains the following hormones and metabolites:

Progesterones

• Progesterone
• 5a-Pregnanediol (5A-PD)
• 5b-Pregnanediol (5B-PD)
• Allopregnanolone
• 21-Hydroxyprogesterone
• 17-Hydroxyprogesterone
• 5-Pregnanetriol

Ratios & Calculations

• alpha vs beta metabolism (5A-PD:5B-PD ratio)

Adrenal Hormones & Metabolites

Free Cortisol & Cortisone

• Cortisol Waking
• Cortisol Waking +2 hours
• Cortisol Dinnertime
• Cortisol Bedtime
• Cortisol/day (F) (total)

• Cortisone Waking
• Cortisone Waking +2 hours
• Cortisone Dinnertime
• Cortisone Bedtime
• Cortisone/day (E) (total)

• Creatinine Waking
• Creatinine Waking +2 hours
• Creatinine Dinnertime
• Creatinine Bedtime
• Creatinine/day (total)

Corticoid Metabolites and DHEA

• Corticosterone
• Tetrahydrodehydrocorticosterone
• 5b-Tetrahydrocorticosterone
• 5a-Tetrahydrocorticosterone
• 11-Deoxycortisol
• 5a-Tetrahydrocortisol
• 5b-Tetrahydrocortisol
• Tetrahydrocortisone
• Dehydroepiandrosterone
• Dehydroepiandrosterone Sulfate

Ratios & Calculations

• Total DHEA (DHEA+DHEAS)
• Metabolized Cortisol (THE+5a-THF+5b-THF)
• Cortisol/Cortisone Metabolites ((5a-THF+5b-THF)/THE)
• 11b-HSD activity (Cortisol/Cortisone metabolites)
• alpha vs beta metabolism (5a-THF/5b-THF ratio)

Androgens

• Androstenedione (A4)
• EPI-Testosterone (EPI-T)
• Testosterone (T)
• Androsterone (AN)
• 11-hydroxy-Androsterone (OHAN)
• 5a-Androstanediol (5a-AD)
• 5a-Dihydrotestosterone (5a-DHT)
• Etiocholanolone (ET)
• 11-hydroxy-Etiocholanolone (OHET)
• 5b-Androstanediol(5b-AD)
• Dehydroepiandrosterone (DHEA)
• Dehydroepiandrosterone Sulfate (DHEA-S)

Ratios & Calculations

• DHEA+DHEAS (total DHEA)
• 5a reductase activity (androsterone (5a activity)/eticholanolone (5b activity))
• Testosterone/EPI-Testosterone

Estrogens

• Estrone (E1)
• 2-Hydroxyesterone (2-OH-E1)
• 4-Hydroxyestrone (4-OH-E1)
• 16a-Hydroxyestrone (16-OH-E1)
• 2-Methoxyestrone (2-M-E1)
• 4-Methoxyestrone (4-M-E1)

• Estradiol (E2)
• 2-Hydroxyestradiol (2-OH-E2)
• 4-Hydroxyestradiol (4-OH-E2)
• 2-Methoxyestradiol (2-M-E2)
• 4-Methoxyestradiol (4-M-E2)

• Estriol (E3)

Ratios & Calculations

• 2-OH-E1 %
• 4-OH-E1 %
• 16-OH-E1 %
• 2-M-E1:2-OH-E1 (COMT/Methylation activity)
• 2-M-E2:2-OH-E2 (COMT/Methylation activity)
• 4-M-E1:4-OH-E1(COMT/Methylation activity)
• 4-M-E1:4-OH-E2(COMT/Methylation activity)
• 2-OH-E1:16-OH-E1 (2:16 OH ratio)
• 4-OH-E1:2-OH-E1 (4:2 OH ratio)

Oxidative Stress

• 8-hydroxy-2-deoxyguanosine

This urine profile can be used in place of blood testing; however, it is ideal to use in combination with blood tests for hormones, especially for more complex hormone issues since it captures more hormones and metabolites than most blood tests.

Collection Notes: This 4-5 sample collection is performed over 2 days, starting with a dinnertime collection and ending with a collection 2-3 hours post-waking.

• The first two collections are performed the evening of day 1 (before dinner and at bedtime).
• The last two collections are performed the morning of day 2 (upon waking, and 2-3 hours post-waking). 
• The fifth collection is only required if you wake up mid-sleep between days 1 and 2. If you do not wake up mid-sleep, you should not collect this extra sample.

Please review the collection instructions carefully and plan ahead for your collection. Caffeine, alcohol, tobacco, and nicotine products should be avoided for 24 hours prior to and during the collection period.

Processing Laboratory: Doctor’s Data
Turnaround Time: 1-2 weeks from date received.
Restrictions: No kits available in PA, AK, or HI. This test is not available in NY.